First Identification of the Effects of Low Frequency Electromagnetic Field on the Micromolecular Changes in Adipose Tissue-Derived Mesenchymal Stem Cells by Fourier Transform Infrared Spectroscopy

" The first response of cells is on the micromolecular level, which is not even visible in functional or morphological levels, sometimes not even in protein or gene expression levels. During this very dynamic molecular stage, many processes including those reversible, have taken place. FTIR gives the possibility to monitor early subtle changes, which may manifest later during the process of adaptation, or when stem cells changes its status. These afterward changes can be seen on the epigenome, transcriptome, proteome level, and so on. From currently available literature, we know that EMF strongly influences many internal mesenchymal stem cell processes such as proliferation, viability, cell cycle, carcinogenesis, differentiation, and death." {Credits 1}

" FTIR spectra show differences between samples under the influence of EMF before they will be manifested at the morphological level. Presented analyzes support our supposition that EMFs affect adipose tissue-derived mesenchymal stem cells. The largest differences in the range of nucleic acids and proteins in the fingerprint region occurred at 24 and 48 h of EMF exposure. That means that during the first 48 h, when a great number of dynamic changes occurred, EMF affects micromolecular composition of proteins, fats, and nucleic acids and therefore changes their structure. However, in the case of AT-MSCs in 72 h EMF and 72 h control, no significant differences were noted in the FTIR spectra, which means that the chemical composition in these two cases is similar. EMF is not neutral for stem cells, especially in the in the first hours of interaction (24 h, 48 h)." {Credits 1}

{Credits 1} 🎪 Łach, K., Cebulski, J., Chaber, R., Kocan, B., Wojnarowska-Nowak, R., & Banaś-Ząbczyk, A. (2021). First Identification of the Effects of Low Frequency Electromagnetic Field on the Micromolecular Changes in Adipose Tissue-Derived Mesenchymal Stem Cells by Fourier Transform Infrared Spectroscopy. Journal of medical physics, 46(4), 253–262. https://doi.org/10.4103/jmp.jmp_57_21. © 2021 Journal of Medical Physics. This article is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License.