The Influence of Burst-Firing EMF on Forskolin-Induced Pheochromocytoma (PC12) Plasma Membrane Extensions

The study shows that the specific pattern of electromagnetic field is more influential than its intensity or duration (in this case to enhanced neurite formation as measured by the proportion of treated cells that expressed PME or multipolar extensions):

" The results of these studies show that there is a specificity of the applied EMF pattern in order to appreciably alter the proportion of observed PME as compared to chemical only treatment. For example, exposure to the Burst-firing EMF was the most successful at increasing the proportion of PME-bearing cells and the proportion of cells with multipolar extensions. Exposure to the Thomas-EMF and the Reverse-Burst-EMF did not affect PC12 morphology. The observation that the inverted presentation of the Burst-firing EMF, reverse-Burst, was no longer able to promote neuritogenesis suggests that simply supplying the same energy to the system is not sufficient to affect PC12 cells under these conditions but the pattern of the EMF is critical in mediating its effects. Exposure to the 7-Hz pulse was also able to increase the proportion of PME-bearing cells, similar to the Burst-firing pattern." {Credits 1}

{Credits 1} 🎪 Carniello, T.N.; Lafrenie, R.M.; Dotta, B.T. The Influence of Burst-Firing EMF on Forskolin-Induced Pheochromocytoma (PC12) Plasma Membrane Extensions. NeuroSci 2021, 2, 383-399. https://doi.org/10.3390/neurosci2040028. © 2021 The Author(s). This article is licensed under the Creative Commons Attribution License.