" .. effects observed rely upon the specific pattern used. In this way, it is worth noting that Procedure #1 (10-10-10; L-phase: 10 min, D-phase: 10 min and L-phase: 10 min) was the most effective. In contrast, patterns with longer exposure times to light, such as Procedures #2 (15-10-15) and #3 (20-10-20) had less effect. Additionally, our preliminary experiments conducted before setting the experimental conditions also showed that continuous light-exposure patterns without a D-phase, of 5 min, 10 min, 15 min and 20 min of continuous L-phase, were much less effective than the 10-10-10 photo-stimulation pattern. These data clearly point out that the improving effect on boar sperm function induced by red LED-based light depends on the photo-stimulation pattern. A similar phenomenon has been described when laser systems are applied to sperm from other mammalian species like dog, buffalo and human25,32,33. Therefore, it seems that light-effects on mammalian sperm rely on precise rhythms and rates of application, regardless of light source and wavelength range."
Last modified on 12-Jan-17